- AOCE 2026

Abstract Submission

Abstract Title

Anti-obesity Properties of Parkia speciosa Pod Extract and Its Effect on Adipogenic Protein Expressions In 3T3-L1 Cells

Presentation Type

Poster Presentation

Type Reference

Scientific Research Abstract

Abstract Category

Obesity

Author's Information

Number of Authors (including submitting/presenting author) *

No more than 15 authors can be listed (as per the Good Publication Practice (GPP) Guidelines).
Please ensure the authors are listed in the right order.

Co-author 1

Nur Dayana Hassan Cheong dayanacheong@gmail.com Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences 42300 Puncak Alam, Selangor Malaysia *

Co-author 2

Emida Mohamed emida894@uitm.edu.my Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences 42300 Puncak Alam, Selangor Malaysia -

Co-author 3

Norhisham Haron hishamharon@uitm.edu.my Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences 42300 Puncak Alam, Selangor Malaysia -

Co-author 4

Siti Nazrina Camalxaman sitinazrina@uitm.edu.my Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences 42300 Puncak Alam, Selangor Malaysia -

Co-author 5

Co-author 6

Co-author 7

Co-author 8

Co-author 9

Co-author 10

Co-author 11

Co-author 12

Co-author 13

Co-author 14

Co-author 15

Abstract Content

Background and aims *

Obesity being a major health concern worldwide has led to an increased interest towards herbal medicines due to their natural origin, minimal side effects and cost-effectiveness. Parkia speciosa is commonly reported for its therapeutic effects in antimicrobial, anticancer, and antidiabetic treatments. However, its anti-obesity effects have not yet been explored. This study aims to investigate the antioxidant properties of P. speciosa pod extract (PSPE) and its anti-adipogenic potential in reducing the adipogenic proteins in 3T3-L1 adipocytes.

Methods *

In this study, the antioxidant capacity of PSPE was evaluated using the DPPH and FRAP assays. The effect of PSPE (3.91 – 1000µg/mL) on 3T3-L1 cell viability was evaluated by using MTT assay. To determine the anti-adipogenic potential of PSPE, the cells were treated with optimum concentrations of extract for 15 days and Oil Red O staining as well as triglyceride assay were performed. Simvastatin-treated cells was used as the control group. The reduction in C/EBPb, C/EBPd, PPARg, and C/EBPa in 3T3-L1 cells were analysed using SDS-PAGE and Western Blot.

Results *

In DPPH assay, the IC50 value for PSPE was 57.99 ± 5.62µg/mL, almost twice that of ascorbic acid. Similarly, the FRAP value for 31.25µg/mL PSPE was 46.83 ± 2.31µg FeSO4/mL, approximately half that of ascorbic acid. The FRAP values of the extract increased in a dose-dependent manner. MTT assay showed that the concentrations of PSPE at 15.63 and 31.25µg/mL both successfully maintained cell viability at >80%. Cells treated with 31.25µg/mL PSPE showed a 20.38% reduction in lipid accumulation, while 15.63µg/mL PSPE exhibited ~10% (p<0.05). For triglyceride assay, significant reductions were observed in cells receiving 31.25µg/mL PSPE at 46.66%, while 15.63µg/mL PSPE reduced 18.68% (p<0.05). The reduction in lipid accumulations were reflected by the significant downregulation of C/EBPb, C/EBPd, PPARg, and C/EBPa proteins expressions (p<0.05).

Conclusions *

In conclusion, the findings of this study strongly indicate that PSPE effectively reduces lipid accumulation and triglyceride levels in 3T3-L1 adipocytes by downregulating proteins involved in adipocyte differentiation and lipid metabolism. Therefore, these results suggest that Parkia speciosa pod extract may serve as a promising natural agent for the treatment of obesity.

Keyword(s)

Adipogenesis, Extract, Obesity, Parkia speciosa, Protein Expression

Figure 1

https://storage.unitedwebnetwork.com/files/1305/da6b2262e6d1d25b4563f20e03178c97.png

Figure 1 Caption

The effects of PSPE and simvastatin treatments on (a) C/EBPβ (b) C/EBPδ (c) PPARγ (d) C/EBPα protein expressions in 3T3-L1 adipocytes. Data are expressed as the mean ± SEM of three independent experiments (n = 3).